Name: Alkaline Phosphatase (ALP) Assay Kit | Liver &amp; Bone Diagnostic Reagent
SKU: 1131
Availability: InStock

Alkaline Phosphatase (ALP) Assay Kit | Liver & Bone Diagnostic Reagent

Precision Enzymatic Diagnostic Solution

Clinical Excellence: A primary marker for evaluating hepatobiliary diseases (cholestasis) and bone disorders (Paget’s disease).
IFCC Standardized: Formulated according to the International Federation of Clinical Chemistry (IFCC) recommendations for global accuracy.
Ready-to-Use Liquid Reagents: Liquid-stable format reduces preparation time and eliminates reconstitution errors.
High Sensitivity: Capable of detecting subtle enzymatic changes with a low detection limit of 5 U/L.
Rapid Kinetic Assay: Provides results in minutes via a continuous monitoring “increase” reaction.
Automation Compatible: Seamlessly integrates with semi-auto and fully automated biochemistry analyzers.

Description

Alkaline Phosphatase (ALP) Assay Kit: The Essential Bio-Marker for Multi-Organ Health

Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides and proteins. Found most concentrated in the liver, bile ducts, and bones, ALP levels are the diagnostic “gold standard” for identifying biliary obstructions and bone-forming activity. Our ALP Assay Kit utilizes a kinetic colorimetric method to provide laboratories with high-speed, reliable data.

Diagnostic Power: Liver vs. Bone

An ALP test is vital for clinicians when patients present symptoms of liver or bone disease.

Hepatobiliary Monitoring: Highly elevated ALP levels often indicate blocked bile ducts (obstructive jaundice), cirrhosis, or liver cancer.
Bone Health Assessment: Increased levels are observed during bone healing, Paget’s disease, rickets, and osteomalacia due to increased osteoblastic activity.
Pediatric Growth: The kit is calibrated to handle the higher physiological ALP levels typically found in growing children and adolescents.

Key Technical Advantages

Optimized Buffer System: Uses an AMP (2-amino-2-methyl-1-propanol) buffer to maintain the ideal pH (10.4) for maximum enzyme catalytic activity.
Metal Ion Activation: Contains specific concentrations of Zinc and Magnesium ions, which act as essential cofactors to ensure full enzymatic recovery.
Minimal Interference: Advanced formulation protects against common interferences such as Bilirubin (up to 40 mg/dL) and Hemoglobin.
Kinetic Precision: Measures the rate of p-Nitrophenyl phosphate conversion to p-Nitrophenol at 405 nm, ensuring linear and reproducible results.

Technical Specifications Table

Feature	Technical Specification
Methodology	Kinetic Colorimetric (IFCC Recommended)
Wavelength	405 nm
Reagent Type	Liquid Stable (R1 + R2)
Incubation Temp	37°C (Standard)
Linearity Range	5 U/L to 800 U/L (Extendable via dilution)
Storage Conditions	2°C to 8°C (Protect from light)
Sample Type	Fresh Serum or Heparinized Plasma
Shelf Life	12 – 18 Months from Manufacture

The InnerWorld Commitment

InnerWorld is Pakistan’s trusted partner for high-precision diagnostic reagents. Our ALP Assay Kits are stored and transported under strict temperature-controlled conditions to preserve enzymatic integrity. We offer full technical support, including Instrument Application Programming for various automated platforms, ensuring your lab gets accurate results from day one.

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