Description
AST Assay Kit: Essential Diagnostics for Liver and Heart Health
Aspartate Aminotransferase (AST), formerly known as Serum Glutamic-Oxaloacetic Transaminase (SGOT), is an enzyme found in high concentrations in the liver, heart, and skeletal muscles. When these tissues are damaged, AST is released into the bloodstream. Our AST Assay Kit provides a professional-grade, kinetic UV method to quantify this enzyme’s activity, aiding in the diagnosis of myocardial infarction, viral hepatitis, and liver cirrhosis.
Clinical Significance of AST Testing
While often paired with ALT to calculate the De Ritis Ratio, AST is uniquely sensitive to cellular necrosis. Elevated levels are frequently associated with:
- Hepatocellular Injury: Monitoring the progression of chronic hepatitis and toxic liver damage.
- Myocardial Infarction: AST levels rise significantly following heart muscle damage.
- Muscle Disorders: Helpful in diagnosing skeletal muscle trauma and muscular dystrophy.
Key Technical Advantages
- Kinetic UV Measurement: Utilizes the Malate Dehydrogenase (MDH) coupled reaction for the most accurate and interference-free measurement at 340nm.
- Lipid Clearing Technology: Advanced surfactants prevent interference from lipemic samples, ensuring clarity and precision.
- Minimized Substrate Exhaustion: High-purity NADH ensures a wide dynamic range, reducing the need for sample dilution.
- Optimized Buffer System: Provides a stable pH environment to maintain enzyme activity during the entire reaction phase.
Technical Specifications Table
| Feature | Technical Specification |
| Common Name | AST / SGOT Assay Kit |
| Methodology | Kinetic UV (IFCC standardized) |
| Wavelength | 340 nm |
| Reagent Format | Liquid Stable (R1 + R2) |
| Linearity | Up to 600 U/L (Depending on analyzer) |
| Sensitivity | 1.0 U/L |
| Storage Temperature | 2°C to 8°C (Protect from light) |
| Sample Volume | 50 µL to 100 µL (Typical) |
| Compatibility | Fully & Semi-Automated Analyzers |
Trusted Medical Partner: InnerWorld Global
At InnerWorld, we specialize in the distribution of high-quality clinical reagents across Pakistan. Every AST Assay Kit we supply is backed by a comprehensive Certificate of Analysis (CoA) and expert technical support. Our cold-chain logistics ensure that your reagents maintain maximum enzymatic activity from our warehouse to your laboratory.



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