Alanine Aminotransferase (ALT) Assay Kit | Liver Function Diagnostics

Description

Alanine Aminotransferase (ALT) Assay Kit: The Gold Standard in Hepatic Health Monitoring

Alanine Aminotransferase (ALT), also known as Serum Glutamic-Pyruvic Transaminase (SGPT), is a critical enzyme found predominantly in the liver. When liver cells are damaged or inflamed, ALT is released into the bloodstream, making its measurement the primary clinical indicator for liver dysfunction. Our ALT Assay Kit provides a robust, simple, and direct procedure for quantifying enzymatic activity in various biological samples.

[Image: Diagnostic Liver Panel Chart showing ALT/AST relationship]

Detection Principle & Scientific Methodology

The assay follows a coupled enzymatic reaction. ALT catalyzes the transfer of an amino group from L-alanine to α-ketoglutarate, generating Pyruvate and Glutamate. In the colorimetric method, pyruvate reacts with a specific probe to produce a colored product (measurable at 510–570 nm). In the UV method, the conversion of pyruvate consumes NADH, resulting in a decrease in absorbance at 340 nm. This rate of decrease is directly proportional to the ALT activity in the sample.

Key Features & Technical Advantages

1. Multiple Detection Modes: Available in both Colorimetric and UV formats to suit your laboratory’s existing spectrophotometric equipment.

2. Wide Linear Range: Capable of detecting ALT levels from low-concentration pediatric samples to highly elevated clinical markers (up to 1000 U/L).

3. Inhibitor-Free Buffer: Optimized buffer systems ensure no interference from endogenous keto acids or chemical inhibitors.

4. Minimal Sample Volume: Requires only 5–20 µL of sample, preserving precious clinical specimens.

Technical Specifications Table

Trusted Laboratory Partner: InnerWorld Global

InnerWorld is committed to providing premium diagnostic reagents that bridge the gap between quality and affordability. Our ALT Assay Kits are sourced from ISO-certified manufacturing facilities and undergo rigorous quality control. We provide full Technical Support, Safety Data Sheets (SDS), and Certificate of Analysis (CoA) with every batch to ensure your laboratory operates at the highest standards.